Detail Cloning and expression of MPT83 gen from Mycobacterium tuberculosis local strain in E.coli BL21 as vaccine candidate of tuberculosis Bibliografi Author: Natzir, Rosdiana ; Ardyansyah, Bau Dilam ; Massi, Muh. Nasrum ; Agus, Rosana ; Ahmad, Ahyar Topik: Tuberculosis; MPT83 antigen; recombinant plasmid; Cloning; GST-MPT83 Bahasa: (EN )     Penerbit: Fakultas Kedokteran Universitas Hasanuddin     Tempat Terbit: Ujung Pandang    Tahun Terbit: 2014     Jenis: Article - diterbitkan di jurnal ilmiah nasional Fulltext: FINAL JURNAL Mpt83 par same 2.pdf (363.94KB; 1 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract The appearance of Mycobacterium tuberculosis strains leading to drug resistance has caused new problems in TB treatment in various parts of the world and forces WHO to declare TB as a global emergency. With the increase of TB drug resistance, it is convinced that a more effective vaccine development will stop or decrease the epidemic of TB. Some M. Tuberculosis antigens, one of which is MPT8, have been examined as TB vaccine candidate, using DNA recombinant technology. MPT83 antigen, which is very immunogenic in lipoprotein micro bacteria, is identified as surface cell interrelated to antigen with electron microscope and cytometry circulation. MPT83 can also stimulate humoural as well as T-cell immune response. Next, MPT83 is an M. tuberculosis surface antigen that can induct CD4 lymphocyte T in order to produce Interleukin-2 that has a central role in triggering immune against TB. Having TB resistance from BCG vaccine, MPT83 is considered TB vaccine candidate that can protect people against TB at adult age. The purpose of the research is to conduct amplification of MPT83 antigen cloning, and expression of its antigen on E coli bacteria. From the result of the research, it is expected that raw material to produce TB vaccine as well as a high-quality antigen can be obtained. The band of DNA in PCR product is 660 bp, while the one in pGEMT-Easy -MPT83 recombinant plasmid is 3678 bp. This is expressed in BL21 E coli strain and produces 44 kDa protein as well as GST-MPT83 fusion protein. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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