Effect of Beta2glycoprotein I and Human Monoclonal Anticardiolipin Antibody on The Protein S/C4b-Binding Protein System  

Oleh:

Atsumi, T. ; Khamashta, MA ; Ames, Prj ; Ichikawa, K. ; Koike, T. ; Hughes, GRV

Jenis: Article from Journal
Dalam koleksi: Lupus vol. 6 no. 4 (1997), page 358-364.
Topik: Antiphospholipid Antibody; Antiphosolipid Syndrome; Coagulation; Thrombosis
Fulltext: 358.full.pdf (649.33KB)

Isi artikel

The effect of β2glycoprotein I (β2GPI) and human monoclonal anticardiolipin antibody (aCL) on the protein S/C4b-binding protein (C4BP) system was evaluated. The binding of C4BP to protein S was assessed by ELISA in the presence of β2GPI with/without human monoclonal aCL. β2GPI downregulated the binding between S and C4BP significantly. Human monoclonal aCL abolished the β2GPI inhibitory effect in a calcium (+)C+a independent fashion. In separate experiments, the reactivity of aCL towards protein S in the presence or absence of β2GPI and cardiolipin was investigated. Monoclonal aCL bound to protein S only in the presence of a combination of β2GPI and cardiolipin. This binding was C +a+ dependent. These findings suggest that human monoclonal aCL increases the affinity of C4BP for protein S, and that protein S may represent one of the targets for aCL when combined with β2GPI and cardiolipin. Both issues may explain acquired free protein S deficiency and the attendant risk of thrombosis in patients with aCL.

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