In Vitro Type-1 and Type-2 Cytokine Production in Systemic Lupus Erythematosus: Lack of Relationship with Clinical Disease Activity  

Oleh:

Barcellini, W ; Rizzardi, GP ; Borghi, MO ; Nicoletti, F ; Fain, C ; Papa, N Del ; Meroni, PL

Jenis: Article from Journal
Dalam koleksi: Lupus vol. 5 no. 2 (1996), page 139-145.
Topik: SLE; SCD23; Cytokines; Polyclonal B Cell Activation
Fulltext: 139.full.pdf (619.78KB)

Isi artikel

Objective: to investigate the relationship between disease activity and in vitro cytokine, soluble(s)CD23 and polyclonal and anti-DNA antibody production by PBMC from patients with active systemic lupus erythematosus (SLE). Methods: cytokines, sCD23 and immunoglobulins were estimated by ELISA in unstimulated and polyclonal mitogen-stimulated culture supernatants. Results: PHA-induced IL-2 and IFN-y production were decreased, whereas spontaneous and PHA-induced IL-6 and IL-10 production were increased in cultures of SLE lymphocytes. Conversely, spontaneous and PHA-stimulated IL-4 and sCD23 production was comparable between patients and controls. Finally, we found an increase in in vitro spontaneous polyclonal and anti-DNA IgG secretion. Conclusions: We demonstrated an expanded type-2 cytokine profile with no correlation with parameters of disease activity.

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