Adoptive transfer of mFas ligand into dendritic cells influences the spontaneous resorption rate in the CBA/J × DBA/2 mouse model  

Oleh:

Zhao, Aimin ; Miao, Xiong ; Yu, Zhang ; Shimin, Bao ; Jian, Zhang ; Lihua, Qiu ; Qide, Lin

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 93 no. 05 (Mar. 2010), page 1700-1705.
Topik: Adoptive transfer; DC; embryo resorption rate; FasL; spontaneous abortion

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2010.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To investigate the effect of mFasL dendritic cells (DC) on the embryo resorption rate in the CBA/J × DBA/2 abortion mouse model. Design Experimental study. Setting University hospital in a major city in China. Animal(s) 101 CBA/J mice and 50 DBA/2 mice. Intervention(s) We constructed the eukaryotic expression vector pcDNA3.1-mFasL, derived the DCs from bone marrow of DBA/2 mice, and transfected the DCs with pcDNA3.1-mFasL. The abortion mouse model were established by mating female CBA/J mice with DBA/2 mice. Via the CBA/J × DBA/2 abortion mouse model, five groups were established: group 1: abortion model without treatment; group 2: abortion mouse model injected with DC culture medium (DCCM); group 3: abortion mouse model injected with DC; group 4: abortion mouse model injected with empty plasmid pcDNA3.1-DC; group 5: abortion model mouse injected with pcDNA3.1-FasL-DC. Main Outcome Measure(s) Comparison of difference in the embryo resorption rates of the CBA/J × DBA/2 abortion mouse model treated with either pcDNA3.1-mFasL-DC or different controls observed on gestation days 12 to 14. Result(s) The embryo resorption rate was statistically significantly decreased in group 3 treated with DC and group 4 with empty plasmid pcDNA3.1-DC when they compared with group 1 (no treatment for abortion mouse model) and group 2 (injected with DC culture medium, DCCM). Furthermore, abortion model group 5 (injected with pcDNA3.1-mFasL-DC) showed a statistically significantly decreased in embryo resorption rate when compared with the other four groups, including groups 1 and 2 and groups 3 and group 4. Conclusion(s) Adoptive transfer of mFasL-DC can statistically significantly reduce the embryo resorption rate in the CBA/J × DBA/2 abortion mouse model.

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