Detail Peningkatan Produksi Azadirahtin dalam Kultur Suspensi Sel Azadirachta indica A.Juss melalui Penambahan Skualen (artikel di dalam Jurnal Matematika dan Sains Vol. 8 No. 4, Desember 2003, hal 141 – 146) Bibliografi Author: Zakiah, Zulfa ; Marwani, Erly ; Siregar, Arbayah H. Bahasa: (ID )     Penerbit: Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Indonesia     Tempat Terbit: Bandung    Tahun Terbit: 2003     Jenis: Article - diterbitkan di jurnal ilmiah nasional Fulltext: 212-620-1-PB.pdf (145.42KB; 6 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract One of the methods to enhance the secondary metabolite content in plant tissue culture is precursor feeding. The study on azadirachtin enhancement in cell suspension culture of Azadirachta indica A.Juss was conducted by supplying squalene as precursor. Callus were induced on Murashige-Skoog solid medium with addition of 0,5µM 2,4-dichlorophenoxy acetic acid (2,4-D) and 1,0µM 6-benzylaminopurine (BAP). Callus were subcultured to Murashige-Skoog liquid medium with addition of 0,1µM 2,4-D and 1,0µM BAP. The cell suspension cultures were subcultured three times, and then treated each with 10, 100, and 1000µM of squalene. Azadirachtin content was measured at 0, 2, 4, 6, 8, 10 and 12 days after feeding using HPLC (High Pressure Liquid Chromatography) with methanol:water (6:4) as mobile phase. The results showed that the highest azadirachtin content in cell suspension culture before supplying squalene was 0,041 g/g cell dry-weight (dw) at the age of 8 days culture. The increment of azadirachtin content in cell and medium was significantly influenced by squalene concentration and age of culture. The highest azadirachtin content after feeding in the cell mass (0,076 ± 0,006 g/g dw) and in the medium (0,229 ± 0,003 mg/L)was achieved on supplying 100µM squalene to 10 and 12 days culture. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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