| Bisbul (Diospyros blancoi) is a kind of edible fruit and could be beneficial as inhibitor for skin ageing process. Majority of root plants have symbiotic associations with arbuscular mycorrhizal fungi (AMF); however, AMF in bisbul has never been reported. The objective of this study was to observe AMF colonies and to identify AMF spores in bisbul tree rhizospheres and in pot-cultured Pueraria javanica. Roots and soil samples from bisbul rhizospheres were collected from three locations in Bogor area. Roots were stained using trypan blue 0.05%. Soil samples were air dried, then part of them were used for spore isolation and the rest were used for pot cultures. Spores were isolated by wet sieving and decanting method and then continued with centrifugation. The results showed that the structures of AMF colonies in bisbul roots were arbuscules, vesicles, coiled hyphae, and internal hyphae. Isolated AMF spores were identified as Acaulospora longula, A. scrobiculata, A. tuberculata, Claroideoglomus geosporum, Funneliformis etunicatum, Gigaspora candida, G. ramisporophora, Glomus albidum, G. glomerulatum, and Scutellospora calospora. This is the first report of mycorrhizal infection in the root of bisbul and AMF association with bisbul root. |