Effect of Myogenic Stem Cells on Contractile Properties of the Repaired and Unrepaired Transected Esternal Anal Sphincter in an Animal Model  

Oleh:

White, Amanda B ; Keller, Patrick W. ; Acevedo, Jesus F. ; Word, R. Ann ; Wai, Clifford Y.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Obstetrics and Gynecology vol. 115 no. 04 (Apr. 2010), page 815-823.
Topik: OBSTETRI GINEKOLOGI

Ketersediaan

Perpustakaan FK Nomor Panggil: O01.K.2010.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective: To estimate the effect of myogenic stem cells on contractile function of the external anal sphincter after transaction with or without repair in an animal. Methods: One hundred twenty virginal female rats were randomly assigned to repair (n=60) or no repair (n=60) after anal sphincter transaction. Animals were further divided into two groups: 40-microliter injection at the transaction site with either phosphate-buffered solution (control) or myogenic stem cells (3.2x10 6 cells). Animals were killed at 7, 21, or 90 days, and the anal sphincter complex dissected and analyzed for contractile function. Results: Contractile function of the external anal sphincter was severely impaired 7 days after sphincter transaction with or without repair. Twitch tension, maximal titanic contraction, and maximal contractile force in response to electrical field stimulation improved significantly with time after sphincter repair. Injection of myogenic stem cells in the anal sphincter at the time of repair resulted in superior contractile function at both 7 days and 90 days compared with controls. Intertingly, contractile function of the nonrepaired external anal sphincter did not improve with time with or without myogenic stem cells. Indicators of denervation (fatique and twitch or tetany ratios) did not change among groups. Conclusion: In this animal model, injection of myogenic stem cells at the time of external anal sphincter repair resulted in enhanced contractile function at 90 days compared with repair alone. Without repair, function of the external anal sphincter was not improved by stem cell therapy at any time point. These results suggest that addition of myogenic stem cells improves both acute and long-term function of the external anal sphincter after mechanical injury.

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