Experimental immunological infertility effect of anti-GAPDH-2 antibodies on the fertility of female mice  

Oleh:

Yong, Wang ; Ning, Zhang ; Xiaodong, Zhang ; Shiying, Miaoa ; Shudong, Zongb

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 92 no. 06 (Dec. 2009), page 2020-2027.
Topik: GAPDH-2; sperm antigen; anti-GAPDH-2; anti-sperm antibody (ASA)

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.04 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective To examine the relationship between an antibody against GAPDH-2, a sperm-specific protein, and infertility of female mice. Design Basic research. Setting National Research Institute for Family Planning Beijing, World Health Organization Collaboration Center of Human Reproduction. Animal(s) New Zealand rabbit, NIH and ICR mice. Intervention(s) None. Main Outcome Measure(s) Enzyme-linked immunoabsorbent assay, Western blot and indirect immunostaining assays, standard fertility assay, and sperm agglutination assay. Result(s) Antibodies against the full-length GAPDH-2 were raised. Its specificity was assessed by immunoblotting and indirect immunostaining assays. The antibody immunoreacted with human sperm GAPDH-2 and the mouse homolog GAPDS but did not cross-react with GAPDH. Treatment of female mice with IP injection of anti-GAPDH-2 serum significantly reduced their fertility. Anti-GAPDH-2 serum caused the agglutination of normal mice sperm in vitro. The anti-GAPDH-2 antibody was detectable in the sera and uterine fluid of the mice immunized with GAPDH-2. Conclusion(s) These results show that GAPDH-2 should be further evaluated as a promising candidate in the development of an antifertility immunogen. Detecting anti-GAPDH-2 antibodies in the bodily fluid of subjects afflicted with indeterminate infertility may be a new diagnostic index. Key Words: GAPDH-2, sperm antigen, anti-GAPDH-2, anti-sperm antibody (ASA)

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