Post-biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis  

Oleh:

Polisseni, Juliana ; Ferreira de Sa, Wanderlei ; Guerra, Martha de Oliveira

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 93 no. 03 (Feb. 2010), page 783-788 .
Topik: REPRODUCTIVE HEALTH; PGD; sexing; embryo quality; hatching rate

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Perpustakaan FK Nomor Panggil: F02.K.2010.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Volume 93, Issue 3, Pages 783-788 (February 2010) 16 of 65 ABSTRACT FULL TEXT FULL-TEXT PDF (138 KB) GET FULL TEXT ELSEWHERE CITATION ALERT CITED BY RELATED ARTICLES EXPORT CITATION EMAIL TO A COLLEAGUE RIGHTS/PERMISSIONS DOWNLOAD IMAGES NEED REPRINTS? BOOKMARK ARTICLE Post-biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis Juliana Polisseni, M.S.ab, Wanderlei Ferreira de Sá, Ph.D.a, Martha de Oliveira Guerra, Ph.D.b, Marco Antônio Machado, Ph.D.a, Raquel Varella Serapião, Ph.D.a, Bruno Campos de Carvalho, M.S.c, Luiz Sérgio de Almeida Camargo, Ph.D.a, Vera Maria Peters, Ph.D.b Received 4 July 2008; received in revised form 6 October 2008; accepted 17 October 2008. published online 25 December 2008. Objective To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design Randomized study. Setting Molecular genetics and animal reproduction laboratories. Patient(s) Cow ovaries obtained from slaughterhouses. Intervention(s) The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main Outcome Measure(s) Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s) A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s) Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.

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