High protein intake reduces intrahepatocellular lipid deposition in humans  

Oleh:

Bortolotti, Murielle ; Kreis, Roland ; Debard, Cyrille

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The American Journal of Clinical Nutrition vol. 90 no. 04 (Oct. 2009), page 1002-1010..
Topik: HEALTH AND NUTRITION; increase intrahepatocellular lipids IHCLs; hypercaloric high-fat HF; hypercaloric high-fat; high-protein HFHP

Ketersediaan

Perpustakaan FK Nomor Panggil: A07.K.2009.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Background: High sugar and fat intakes are known to increase intrahepatocellular lipids (IHCLs) and to cause insulin resistance. High protein intake may facilitate weight loss and improve glucose homeostasis in insulin-resistant patients, but its effects on IHCLs remain unknown. Objective: The aim was to assess the effect of high protein intake on high-fat diet–induced IHCL accumulation and insulin sensitivity in healthy young men. Design: Ten volunteers were studied in a crossover design after 4 d of either a hypercaloric high-fat (HF) diet; a hypercaloric high-fat, high-protein (HFHP) diet; or a control, isocaloric (control) diet. IHCLs were measured by 1H-magnetic resonance spectroscopy, fasting metabolism was measured by indirect calorimetry, insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp, and plasma concentrations were measured by enzyme-linked immunosorbent assay and gas chromatography–mass spectrometry; expression of key lipogenic genes was assessed in subcutaneous adipose tissue biopsy specimens. Results: The HF diet increased IHCLs by 90 ± 26% and plasma tissue-type plasminogen activator inhibitor-1 (tPAI-1) by 54 ± 11% (P < 0.02 for both) and inhibited plasma free fatty acids by 26 ± 11% and ß-hydroxybutyrate by 61 ± 27% (P < 0.05 for both). The HFHP diet blunted the increase in IHCLs and normalized plasma ß-hydroxybutyrate and tPAI-1 concentrations. Insulin sensitivity was not altered, whereas the expression of sterol regulatory element-binding protein-1c and key lipogenic genes increased with the HF and HFHP diets (P < 0.02). Bile acid concentrations remained unchanged after the HF diet but increased by 50 ± 24% after the HFHP diet (P = 0.14). Conclusions: Protein intake significantly blunts the effects of an HF diet on IHCLs and tPAI-1 through effects presumably exerted at the level of the liver. Protein-induced increases in bile acid concentrations may be involved.

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