Detail Genetic Diversity of Yeast from Ragi Tape based on 5.8S rDNA-ITS region (in The 5 Indonesia Biotechnology Conference: An International Forum "Green Industrial Innovation through Biotechnology", Mataram, July 4th-7th 2012) Bibliografi Author: Barus, Tati ; Sulistyani, Theresia Topik: genetic diversity; yeast; ragi tape; 5.85 rDNA Bahasa: (EN )     Tahun Terbit: 2012     Jenis: Papers/Makalah - pada seminar internasional Fulltext: 1.pdf (197.04KB; 7 download) Abstract Tape is a well known fermentation food product from Indonesia. Ragi tape is an essential component in tape production because it contains a variety of microbes that play a role as starter culture. One of them is yeast which is the main microbe in tape fermentation. However, study of genetic diversity from yeast is very rare. In this study we analyze genetic diversity of yeast from ragi tape based on DNA sequence of 5.8S rDNA-ITS regions. Samples of ragi tape were collected from a traditional market from different parts of Indonesia. Yeast were grown by using Yeast Mold Agar medium. Sequence of 5.8S rDNA-ITS regions was amplified by using the primers ITS1 and ITS4. Thirty five isolates of yeasts was successfully isolated from sample. Phylogenetic tree performed the 35 isolates consisted of 3 clusters. Based on sequence of 5.8S rDNA-ITS region, cluster 1 consists of Torulaspora delbrueckii, Issatchenkia orientalis, and Trichosporon dermatis. Cluster 2 was Pichia jadinii and cluster 3 was P. kudriavzevii Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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