Antibacterial activity of temu kunci tuber (kaempheria pandurata) essential oil against Bacillus cereus  

Oleh:

Miksusanti ; Laksmi J, Betty Sri ; Syarief, Rizal ; Pontjo, Bambang ; Mulyadi, Gabot Tri

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Medical Journal of Indonesia vol. 18 no. 01 (Jan. 2009), page 10-17.
Topik: leakage; morphology

Ketersediaan

Perpustakaan FK Nomor Panggil: M35.K.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Aim: The mechanism oftemu kunci tuber essential oil potential as antimicrobial agent has not been reported. To analyze the mechanism of antibacterial activity of temu kunci tuber essential oil from Yogyakarta on B.cereus. Antibacterial activity of essential oil were analyzed for its ability to disrupt bacterial cell membrane, that caused cell leakage and altered the morphology of the bacteria. Leakage was measured by analyzing the Ca+2, K+ ion oufflow using an atomic adsorption spectrometry (AAS), and protein and nucleic acid using an ultraviolet spectrophotometer (UVS) on 280 nrn and 260 nm respectively. Alterations in morphology were assessed using scanning electron microscopy (SEM). Results: Minimum inhibitory concentration (MIC) of temu kunci essential oil on B.cereus was 0.12% (v/v). Treatment of B. cereus using lMIC and 2MlC showed significant leakage compared to control (P<0.05). The K+ and Ca+ ion leakage from the bacterial cells were between 10.32-35.57% and 15.05-41.54% respectively and showed significant difference compared to control (P<0.05). The absorbance observed by UVS for protein and nucleic acid leakage were 0.6330-0.8670 at 280 nm and 0.4320-0.8307 at 260 nm, respectively, and were signlficantly different compared to control (P<0.05). Exposure of I MIC temu kunci essential oil on B.cereus caused thickening as well as irregularities on the cell wall. At 2 MIC cells seemed transparent, empty looking and showed a ghost-like appearance. Conclusion: Kaempheria pondurata essential oil could cause leakage and alter the morphology of the bacteria.

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