| The depletion of fossil fuel availability arouses the attention of the world to initially plant fuel-producing crops that do not compete with food crops, one of them is physic nut or Jatropha curcas. The multiplication of this plant can go through cuttings or seeds, but propagation by cuttings requires large amounts of plant breeding and seed multiplication produce seedlings with high diversity. Propagation by tissue culture technique gives the advantage to produce seedlings in a short time, large amount, uniform, and free from disease. The aim of this research was to obtain a combination of provenance with optimal concentration of benzylaminopurine (BAP) for the axillary shoot multiplication of Jatropha curcas. Propagation was performed in tissue culture laboratory of PT SMART Bogor by using the axillary bud explants from sterile seedlings of three provenances of J. curcas seeds taken from the garden of PT BUMI MAS EKA PERSADA, Cikarang, and inisiated on MS medium containing benzylaminopurine (BAP) concentration of 0, 0.1, 0.3, and 0.5 mg/l for 8 weeks. Data were taken at week-4 and 8 and analysed using completely randomized design with unequal replications using of 4 different levels of BAP and three different provenances of J. curcas. In addition, the best period for culture was 4 weeks, because after week-4, the number of shoots was not much increased and the colour of leaves started to turn yellowish. The best numbers of leaves and shoots at week-4 were obtained from provenance E9. The best number of leaves was obtained from BAP concentrations of 0.1 and 0.3 mg/l with an average of 10.5 leaves per explant, while the number of shoots was achieved by the addition of 0.5 mg/l BAP with an average of 2.75 shoots per explant. |