Bacterial Lipopolysaccharide Induces Transforming Growth Factor b and Hepatocyte Growth Factor Through Toll-like Receptor 2 in Cultured Human Colon Cancer Cells  

Oleh:

Yoshioka, T. ; Morimoto, Y. ; Iwagaki, H. ; Itoh, H. ; Saito, S.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The Journal of International Medical Research vol. 29 no. 05 (Sep. 2001), page 409-420.
Topik: Lipopolysaccharide; transforming growth factor; hepatocyte growth factor; colon cancer

Ketersediaan

Perpustakaan FK Nomor Panggil: J11.K.01-04.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

This study examined, in human cancer cell lines, the pattern of cytokine production stimulated by lipopolysaccharide (LPS), a major component of the outer surface of Gram-negative bacteria, and characterized the expression pattern of CD14, cell surface LPS receptor antigen, and toll-like receptors (TLRs), which appear to be key regulators of the innate immune response system. Two colon cancer cell lines (DLD and LoVo), a hepatocellular carcinoma cell line and a myelomonocytic cell line were incubated with LPS for 0 – 72 h, and transforming growth factor (TGF) b1 and b2, hepatocyte growth factor (HGF) and interleukins 6, 8 and 15 were assayed. The only changes induced by incubation with LPS were significant increases in TGFb1 production at 12 h, and in HGF production at 72 h, in LPS-stimulated DLD cells, and significant increases in TGFb2 production after 12 h and in HGF after 72 h in LoVo cells. Using reverse transcriptase–polymerase chain reaction analysis, expression of CD14 and TLR-2 mRNA was detected in DLD and LoVo cells, and expression of TLR-4 mRNA was detected in PLC/PRF/5 and KG-1 cells. These results suggest that LPS induces TGFb and HGF production mediated by CD14/TLR-2 in cultured human colon cancer cell lines.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali