Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos in Vitro  

Oleh:

Karja, Ni Wajan Kurniani ; Fahrudin, Mokhamad ; Kikuchi, Kazuhiro

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 16 no. 1 (Mar. 2009), page 25-29.
Topik: Iodoacetate; Glucose; Early Stage Embryos; In Vitro Culture
Fulltext: 19.pdf (60.49KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.10 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

In porcine preimplantation embryos, glucose-utilizing pathways are reported active throughout their development. The aim of this study was to test the involvement of glycolysis activity in early stage of porcine embryo development by the addition of iodoacetate, a glycolytic inhibitor, to culture medium. In vitro matured and fertilized oocytes were cultured for the first 2 days in iodoacetate at concentrations of 0,1, or 2.5 miuM. The proportion of viable embryos to the blastocyst stage on day 6 was significantly reduced by 1 miuM iodoacetate and noe of cleaved embrous in Ia-2.5 group develop to the blasyocyst stage (P < 0.05). More than 60% of embryos in Ia-2.5 group were arrested at the two-four-cell stage, and then arrested at the morula stage. These results indicate that glucose plays important roles in supporting the development of early stage of procine embryos and that the inhibition of glucose metabolism may disrupt the management of energy production, leaving the embryos incompetence to develop.

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