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BukuMicrobial Diversity Analysis of Marine Sediments from Jagung island, Do'o island, and Batu Termanu using Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis
Bibliografi
Author: SUTEDJO, SAPUTRA ; Waturangi, Diana Elizabeth (Advisor)
Topik: Microbial Diversity; T-RFLP; Marine Sediment; Coral Triangle Initiative
Bahasa: (EN )    
Penerbit: Fakultas Teknobiologi Unika Atma Jaya (Faculty of Biotechnology Atma Jaya Catholic University of Indonesia)     Tempat Terbit: Jakarta    Tahun Terbit: 2011    
Jenis: Theses - Undergraduate Thesis
Fulltext: Saputra Sutedjo's Undergraduate Theses.pdf (813.35KB; 7 download)
Ketersediaan
  • Perpustakaan Pusat (Semanggi)
    • Nomor Panggil: FTB-218
    • Non-tandon: tidak ada
    • Tandon: 1
 Lihat Detail Induk
Abstract
Microorganisms found in marine sediments have an important role in biogeochemical cycles such as carbon, nitrogen, phosphorus, sulfur, and some heavy metals cycle. The purpose of this study was to obtain information on the diversity of microbial communities in marine sediments from Coral Triangle Initiative (CTI) region which represented by Do’o island and Batu Termanu, Rote island which are located in East Nusa Tenggara (EST) and non-CTI region represented by Jagung island from Kepulauan Seribu associated with physical condition of the environment. Samples were taken from Jagung island, Do'o island, and Batu Termanu. Analysis were done using Terminal Restriction Fragment Length Polymorphism (T-RFLP) with three restriction enzymes: BstUI, HpaII, and RsaI. Jagung Island performed the highest diversity with Shannon and Simpson index of 1.54 and 0.63. The results indicated that bacteria found in sediments were from the Actinobacteria, Bacilli, Bacteroidetes, Cyanobacteria, d-Proteobacteria, Flavobacteria, ?-Proteobacteria, and Mollicutes class. This results can be used as an initial reference for diversity information, environmental condition, and also exploration of their beneficial microbes from these regions.
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