Detail A method for growing a biofilm under low shear at the air–liquid interface using the drip flow biofilm reactor Bibliografi Author: Stewart, Philip S. ; Goeres, Darla M. ; Hamilton, Martin A. ; Beck, Nicholas A. ; Buckingham-Meyer, Kelli ; Hilyard, Jackie D. ; Loetterle, Linda R. ; Lorenz, Lindsey A. ; Walker, Diane K. Topik: Pseudomonas aeruginosa; Bio?lm; drip ?ow reactor (DFR) Bahasa: (EN )     Penerbit: Nature Publishing Group     Tempat Terbit: London    Tahun Terbit: 2009     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: A method for growing a biofilm under low shear at the air–liquid interface using the drip flow biofilm reactor.pdf (345.55KB; 3 download) Abstract This protocol describes how to grow a Pseudomonas aeruginosa bio?lm under low ?uid shear close to the air–liquid interface using the drip ?ow reactor (DFR). The DFR can model environments such as food-processing conveyor belts, catheters, lungs with cystic ?brosis and the oral cavity. The bio?lm is established by operating the reactor in batch mode for 6 h. A mature bio?lm forms as the reactor operates for an additional 48 h with a continuous ?ow of nutrients. During continuous ?ow, the bio?lm experiences a low shear as the media drips onto a surface set at a 101 angle. At the end of 54 h, bio?lm accumulation is quanti?ed by removing coupons from the reactor channels, rinsing the coupons to remove planktonic cells, scraping the bio?lm from the coupon surface, disaggregating the clumps, then diluting and plating for viable cell enumeration. The entire procedure takes 13 h of active time that is distributed over 5 d. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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