Detail Protocol. In vitro screens for quorum sensing inhibitors and in vivo confirmation of their effect (in Nature Protocols VOL.5 NO.2) Bibliografi Author: Bjarnsholt, Thomas ; Gennip, Maria van ; Jakobsen, Tim H. ; Christensen, Louise D. ; Jensen, Peter O ; Givskov, Michael Topik: Quorum Sensing (QS); Quorum Sensing Inhibitors (QSIs); Gram-negative bacteria; green fluorescent protein (GFP); acylhomoserine lactone (AHL)-dependent QS inhibition Bahasa: (EN )     Penerbit: Nature Publishing Group     Tempat Terbit: London    Tahun Terbit: 2010     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: In vitro screens for quorum sensing inhibitors and in vivo confirmation of their effect.pdf (712.53KB; 6 download) Abstract This article will introduce the reader to protocols intended for (i) identification of quorum sensing (Qs) inhibitors (QsIs), (ii) characterization of these compounds in vitro and (iii) evaluation of these compounds in animal models. Traditional antimicrobial drugs are designed against planktonic bacteria and not against bacterial biofilms. In biofilms, bacteria are highly resistant to otherwise lethal treatments and they communicate with each other, thus enabling coordinated group behavior. For many years, we have focused on interference with cell to cell communication, also known as QS, with the aim of disabling the expression of virulence and reduction of antibiotic tolerance. Here we present protocols for screening and testing for acylhomoserine lactone (AHL)-dependent QS inhibition. We also present protocols for the in vivo validation of QSIs as possible drug candidates. The presented methods allow the evaluation of QS inhibition by a potential drug candidate within 2–3 weeks. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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