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BukuIsolation and characterization of biosurfactant/bioemulsifier-producing bacteria from petroleum contaminated sites (in Bioresource Technology 97)
Bibliografi
Author: Batista, S.B. ; Mounteer, A.H. ; Amorim, F.R. ; Totola, M.R.
Topik: Biosurfactants; Bioemulsi?ers; Critical micelle concentration; Emulsion stability; Petroleum contamination
Bahasa: (EN )    
Penerbit: Elsevier     Tempat Terbit: Winsland House I    Tahun Terbit: 2006    
Jenis: Article - diterbitkan di jurnal ilmiah internasional
Fulltext: Isolation and characterization of biosurfactant-bioemulsifier-producing bacteria from petroleum contaminated sites.pdf (317.31KB; 1 download)
Abstract
Biosurfactant-producing bacteria were isolated from terrestrial and marine samples collected in areas contaminated with crude oil or its byproducts. Isolates were screened for biosurfactant/bioemulsi?er production in di?erent carbon sources (glucose, fructose, sucrose and kerosene) using the qualitative drop-collapse test. Glucose produced the highest number of positive results (17 of 185 isolates). All 17 isolates produced emulsions with kerosene and 12 exhibited high emulsion-stabilizing capacity, maintaining 50% of the original emulsion volume for 48 h. Eight of the 17 isolates reduced the growth medium surface tension below 40 mN m-1 with 5 exhibiting this capacity in cell-free ?ltrates. Onset of biosurfactant production di?ered among the isolates, with some initiating synthesis during the exponential growth phase and others after the stationary phase was reached. Increasing temperature from 25 to 35 oC accelerated onset of biosurfactant production in only two isolates while pH (6.5–7.6) had no e?ect in any isolate tested. Isolation from petroleum contaminated sites using the screening protocol presented proved to be a rapid and e?ective manner to identify bacterial isolates with potential industrial applications.
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