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X-gal® inhibits the swarming of Vibrio species (in Journal of Microbiological Methods 66)
Bibliografi
Author:
Kim, Moon-Young
;
Park, Ra-Young
;
Bai, Young-Hoon
;
Chung, Yoon-Young
;
Kim, Choon-Mee
;
Kim, Soo-Young
;
Rhee, Joon-Haeng
;
Shin, Sung-Heui
Topik:
Vibrio
;
X-gal
;
ß-galactosidase
;
Swarming
Bahasa:
(EN )
Penerbit:
Elsevier Butterworth-Heinemann
Tempat Terbit:
Oxford
Tahun Terbit:
2006
Jenis:
Article - diterbitkan di jurnal ilmiah internasional
Fulltext:
X-gal(R)-inhibits-the-swarming-of-Vibrio-species_2006_Journal-of-Microbiological-Methods.pdf
(206.6KB;
2 download
)
Abstract
The expressional levels of genes in swarmer cells can be determined by a simple method using X-gal-containing semisolid agars and lacZ-fusion transcription reporter strains of the genes concerned. However, X-gal alone inhibited the swarming of Vibrio, regardless of their ability to digest X-gal. Moreover, X-gal inhibited the growth of V. vulnificus containing functional lacZ. These effects of X-gal itself should be carefully considered when trying to determine the expression levels of genes in swarming cells using X-gal-containing semisolid agar
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