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Detail
BukuX-gal® inhibits the swarming of Vibrio species (in Journal of Microbiological Methods 66)
Bibliografi
Author: Kim, Moon-Young ; Park, Ra-Young ; Bai, Young-Hoon ; Chung, Yoon-Young ; Kim, Choon-Mee ; Kim, Soo-Young ; Rhee, Joon-Haeng ; Shin, Sung-Heui
Topik: Vibrio; X-gal; ß-galactosidase; Swarming
Bahasa: (EN )    
Penerbit: Elsevier Butterworth-Heinemann     Tempat Terbit: Oxford    Tahun Terbit: 2006    
Jenis: Article - diterbitkan di jurnal ilmiah internasional
Fulltext: X-gal(R)-inhibits-the-swarming-of-Vibrio-species_2006_Journal-of-Microbiological-Methods.pdf (206.6KB; 2 download)
Abstract
The expressional levels of genes in swarmer cells can be determined by a simple method using X-gal-containing semisolid agars and lacZ-fusion transcription reporter strains of the genes concerned. However, X-gal alone inhibited the swarming of Vibrio, regardless of their ability to digest X-gal. Moreover, X-gal inhibited the growth of V. vulnificus containing functional lacZ. These effects of X-gal itself should be carefully considered when trying to determine the expression levels of genes in swarming cells using X-gal-containing semisolid agar
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