The Anti-proliferative Effects of Recombinant Human Lysozyme on Human Gastric Cancer Cells  

Oleh:

Guo, T.K. ; Zhao, X.H. ; Xie, X.D. ; Chen, Z.H.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The Journal of International Medical Research vol. 35 no. 03 (May 2007), page 353-360.
Topik: Anti-proliferative effects; recombinant human lysozyme; human gastric cancer cells

Ketersediaan

Perpustakaan FK Nomor Panggil: J11.K.2007.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

We investigated the anti-proliferative effects of recombinant human lysozyme (rHlys) on gastric cancer cell lines and normal human lung fibroblasts. Using conventional molecular cloning techniques we purified rHlys, which we incubated with cultured cells and measured the effects on cell proliferation and viability. At concentrations of 100 and 1000 micrograms/l, rHlys significantly inhibited the growth of human gastric cancer cell lines. In contrast, 10 and 50 micrograms/l of rHlys stimulated gastric cancer cell growth. None of the concentrations of rHlys affected cell viability. Only the highest concentration of rHlys (1000 micrograms/l) inhibited human lung fibroblast growth. Our results suggest that 100 micrograms/l is the optimum growth inhibiting concentration, which inhibited cancer cell growth but not normal cell growth. Our in vitro findings suggest that genetically engineered rHlys might inhibit human gastric cancer cell proliferation in vivo, so it might warrant further investigation as a potential novel anti-cancer agent.

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