The Role of c-Jun N-terminal Kinases 1/2 in Transforming Growth Factor Beta1-induced Expression of Connective Tissue Growth Factor and Scar Formation in the Cornea  

Oleh:

Chang, Y. ; Wu, X.-Y.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: The Journal of International Medical Research vol. 37 no. 03 (May 2009), page 727-736.
Topik: Cornea

Ketersediaan

Perpustakaan FK Nomor Panggil: J11.K.2009.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Transforming growth factor beta1 (TGF-beta1) plays an important role in corneal scar formation, mediated by connective tissue growth factor (CTGF). This study was designed to investigate the effect of c-Jun N-terminal kinase (JNK) 1 and 2 on TGF-beta1- regulated CTGF gene expression and corneal scar formation in telomerase-immortalized human corneal stroma fibroblast (THSF) cells. The effects of CTGF, TGF-beta1 and a JNK inhibitor (SP600125) on the proliferation of THSF cells were studied using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Both CTGF and TGF-beta1 increased THSF cell proliferation and SP600125 inhibited this effect. Immunofluorescence analysis showed that TGF-beta1 stimulated the activation of JNK1/2 and induced cell migration, effects that were inhibited by SP600125. Analysis by real-time reverse transcription–polymerase chain reaction showed that the mRNA levels of the genes for CTGF, fibronectin and collagen I alpha1 in wound and corneal scar formation were reduced by SP600125 pre-treatment.

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