Detail Apparent Induction of Xylanase by Bacillus pumilus PU4-2 Using Pretreated Substrates Bibliografi Author: Widjaja, Sherly ; Purwadaria, Tresnawati ; Ketaren, Pius Pertumpun Topik: Xylanase; Bacillus pumilus PU4-2; Wheat Pollard; Pretreatment Bahasa: (ID )     Penerbit: Fakultas Teknobiologi Unika Atma Jaya (Faculty of Biotechnology Atma Jaya Catholic University of Indonesia)     Tempat Terbit: Jakarta    Tahun Terbit: 2008     Jenis: Article - diterbitkan di jurnal ilmiah nasional Fulltext: Apparent Induction of Xylanase_Sherly Widjaja.pdf (424.72KB; 2 download) Abstract Bacillus pumilus PU4-2 produces xylanase (~1, 4-D-xylan xylanohydrotase; EC 3.2 1.8) in wheat pollard with high activity. Water and NaOH-soaked pollard were used in this research to enhance the production of assayable enzyme. Enzyme activity was produced in minimal media containing 3% w/v untreated or water or NaOH-soaked pollards in 250 ml flasks incubated on shaker incubator at 30°C and 150 rpm for 36 h. The production was also compared to untreated oatmeal known as an indocer substrate. The highest xylanase activity was obtained by using untreated pollard as a sole carbon source. The enzyme activity was 157 U ml wish specific activity at 718 U mg. Xylanase production using different soaking time for water pretreated pollard also confirmed that untreated pollard was the best induce. The production was not influenced by different water soaking times used to remove reducing sugar. Although pretreatment decreased the reducing sugar, the reduction did not enhance assayable enzyme levels. The production was best induced by the soluble oligosaccharides of untreated pollard. Its conclude that B. pumilus PU4-2 was able to produce xylanase with reducing sugar content up to 660 ppm present in production medium. With this reducing sugar level, repression of enzyme production was not detected in the production medium. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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