Detail The impact of arginine-modified chitosan–DNA nanoparticles on the function of macrophages Bibliografi Author: Liu, Lanxia ; Bai, Yuanyuan ; Song, Chunni ; Zhu, Dunwan ; Song, Liping ; Zhang, Hailing ; Dong, Xia ; Leng, Xigang Topik: DNA Nanoparticle; Macrophages; Cytokines; Chitosan; Arginine; Nanobiotechnology Bahasa: (EN )    Edisi: 2 August 2009     Penerbit: Springer Science & Business Media     Tempat Terbit: New York    Tahun Terbit: 2009     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: fulltext.pdf (281.54KB; 0 download) Abstract It has been demonstrated that incorporation of arginine moieties into chitosan significantly elevates the transgenic efficacy of the chitosan. However, little is known about the impact of arginine-modified chitosan on the function of macrophages, which play a vitally important role in the inflammatory response of the body to foreign substances, especially particulate substances. This study was designed to investigate the impact of argininemodified chitosan/DNA nanoparticles on the function of the murine macrophage through observation of phagocytic activity and production of pro-inflammatory cytokines (IL-1b, IL-6, IL-10, IL-12, and TNF-a). Results showed that both chitosan/DNA nanoparticles and arginine-modified chitosan/DNA nanoparticles, containing 20 lg/mL DNA, were internalized by almost all the macrophages in contact. This led to no significant changes, compared to the non-exposure group, in production of cytokines and phagocytic activity of the macrophages 24 h post co-incubation, whereas exposure to LPS induced obviously elevated cytokine production and phagocytic activity, suggesting that incorporation of arginine moieties into chitosan does not have a negative impact on the function of the macrophages. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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