Vitamin D inhibits myometrial and leiomyoma cell proliferation in vitro  

Oleh:

Blauer, Merja ; Rovio, Paivi H. ; Ylikomi, Timo ; Heinonen, Pentti K.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 05 (May 2009), page 1919-1925.
Topik: Uterine leiomyoma; vitamin D; hypovitaminosis; cell growth; in vitro

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective : To determine the effect of 1,25(OH)2D3 and 25(OH)D3 vitamin D derivates on the growth of leiomyoma and myometrial cells in vitro. Design : In vitro study. Setting : Cell biology research laboratory. Patient(s) : Six premenopausal women with uterine leiomyomas undergoing hysterectomy. Intervention(s) : Samples of leiomyomas and normal myometrial tissue were obtained, and paired cultures were established. Main Outcome Measure(s) : A colorimetric crystal violet assay to determine the effect of 1,25(OH)2D3 and 25(OH)D3 on cell growth. Result(s) : In both myometrial and leiomyoma cells, 0.1 nM physiologic level of 1,25(OH)2D3 inhibited growth by 12% when compared with controls. The growth inhibition was concentration dependent; the highest concentration of 1,25(OH)2D3 (100 nM) inhibited growth by 62% in both cell types. All the differences were statistically significant. A slight stimulation (<4%) of cell proliferation was observed with the lowest 25(OH)2D3 concentrations. When treated with either a 500 nM or 1000 nM concentration of the compound, the growth of both cell types fell to approximately 50% of that of the control cultures, and the level of inhibition with the latter concentration was statistically significant. Conclusion(s) : Both myometrial and leiomyoma cell growth in vitro was effectively inhibited by 1,25(OH)2D3. Vitamin D may play a role in the growth of uterine leiomyomas.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali