Regeneration of spermatogenesis by grafting testicular tissue or injecting testicular cells into the testes of sterile mice: a comparative study  

Oleh:

Saen, Dorien Van ; Goossens, Ellen ; Block, Gert De ; Tournaye, Herman

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 5 Sup (May 2009), page 2264-2272.
Topik: Spermatogonial stem cell; tissue grafting; infertility; testis; cancer; transplantation

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective : To make a comparison between two different approaches—spermatogonial stem cell transplantation and intratesticular grafting, for preservation and reintroduction of spermatogonial stem cells. Design : Prospective experimental study. Setting : Academic medical center and teaching hospital. Patient(s) : N/A. Intervention(s) : Intratesticular transplantation, histologic evaluation of testes. Main Outcome Measure(s) : Testicular weight, amount of green fluorescence in the testis, and immunostaining for green fluorescent protein. Result(s) : In a first experiment donor-derived spermatogenesis was found in 65% of the injected testes (41.8 ± 72.2 mm) compared with 75% of the testes (122.1 ± 45.6 mm) after tissue grafting. In the second series of experiments complete spermatogenesis was found in 75% of the testes after fresh grafting (93.8 ± 21.8 mm) compared with 88% after frozen-thawed tissue grafting (84.8 ± 45.6 mm). Conclusion(s) : Both approaches show that spermatogonial stem cells can successfully be introduced to the testis resulting in spermatogenesis. Tissue grafting produced a larger mean donor colony length and there was no significant difference between colonization efficiency using either fresh or frozen-thawed grafts. In a future clinical setting, grafting would be a simple and efficient way for reintroducing stem cells to the testis.

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