Interleukin-10 attenuates TNF-a–induced interleukin-6 production in endometriotic stromal cells  

Oleh:

Tagashira, Yukiko ; Taniguchi, Fuminori ; Harada, Tasuku ; Ikeda, Ayako

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 5 Sup (May 2009), page 2185-2192.
Topik: Endometriosis; interleukin-10; antiinflammatory cytokine; TNF-a

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective : To determine whether high levels of interleukin (IL)-10 can attenuate the production of tumor necrosis factor (TNF)-a–induced proinflammatory cytokines in endometriotic stromal cells. Design : Prospective study. Setting : Department of Ob/Gyn, Tottori University, Japan. Patient(s) : Thirty-five patients with ovarian endometrioma and ten patients with uterine myoma. Intervention(s) : Endometriotic stromal cells were obtained from chocolate cyst linings of ovaries. Endometrial stromal cells obtained from patient with uterine myoma. Main Outcome Measure(s) : Expression of IL-10 gene in endometriotic or endometrial stromal cells was determined by real-time reverse-transcriptase polymerase chain reaction (RT-PCR). We performed immunohistchemical staining to find the presence of IL-10 and IL-10 receptors 1 and 2. We examined the effects of TNF-a and IL-10 on the expression of IL-6 or IL-8 by real-time RT-PCR and ELISA. We examined the activation of intracellular signal transduction molecules in endometriotic stromal cells by Western blotting. Result(s) : Addition of IL-10 suppressed the expressions of IL-6 induced by TNF-a and IL-10 induced the phosporylation of STAT3 in endometriotic stromal cells. TNF-a induced the expression of phosphorylated ERK1/2, JNK1/2, and I?B. Adding IL-10 suppressed the phosphorylation of these signal molecules. Conclusion(s) : Interleukin-10 attenuates TNF-a–induced IL-6 synthesis via NF-?B and MAPK pathways in endometriotic cells.. Interleukin-10 may play a significant role in the pathogenesis of endometriosis.

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