Inverse regulation of the interferon-? receptor and its signaling in human endometrial stromal cells during decidualization  

Oleh:

Fluhr, Herbert ; Ramp, Kristina ; Krenzer, Stefanie ; Licht, Peter ; Zygmunt, Marek

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 5 Sup (May 2009), page 2131-2136.
Topik: Endometrium; decidualization; implantation; IFN-?R; hCG

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective : To investigate whether human endometrial stromal cells (ESCs) express the interferon-?-receptor (IFN-?R) and whether the process of decidualization or human chorionic gonadotropin (hCG) regulate the IFN-?R and its signaling pathway. Design : In vitro experiment. Setting : Research laboratory at a medical university center. Patient(s) : Premenopausal women undergoing hysterectomy for benign reasons. Intervention(s) : Isolation and incubation of ESCs from hysterectomy specimens with 17ß-estradiol, progesterone, recombinant hCG, and IFN-? as well as an IFN-?R-blocking antibody. Main Outcome Measure(s) : We analyzed IFN-?R and the phosphorylation of signal transducer and activator of transcription 1 (STAT-1) by flow cytometry. We measured IFN-?R and interferon response factor 1 (IRF-1) mRNA using semiquantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR). Result(s) : The IFN-?R is up-regulated in human ESCs during decidualization without affecting the phosphorylation of STAT-1. Stimulation of IRF-1 by IFN-? is reduced in decidualized ESCs. We found that hCG neither regulates the IFN-?R nor its signaling pathway. Conclusion(s) : These results show an inverse regulation of the IFN-?R and its signaling response via STAT-1 and IRF-1 in human ESCs during decidualization. The early embryonic signal hCG has no effect on this process. This mechanism may finely modulate the reactivity of ESCs to IFN-?-mediated signals from immune cells at the implantation site

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