Bacterial endotoxin (LPS)–induced DNA damage in preimplanting embryonic and uterine cells inhibits implantation  

Oleh:

Jaiswal, Yogesh Kumar ; Jaiswal, Mukesh Kumar ; Agrawal, Varkha ; Chaturvedi, Madan Mohan

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 5 Sup (May 2009), page 2095-2103.
Topik: Lipopolysaccharide (LPS); blastocyst; uterus; implantation; pregnancy loss; DNA damage; gram-negative bacterial infection

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective : To investigate lipopolysaccharide (LPS)-induced DNA damage in preimplanting embryonic and uterine cells during preimplantation period of pregnancy that may ultimately inhibit the process of implantation in mouse. Design : Animal study. Setting : Academic research environment. Animal(s) : Sixty four Park strain female mice. Intervention(s) : The “minimum dose” (MD) of LPS was injected intraperitoneally in the pregnant females on day 0.5 of pregnancy, and individual embryos and uterine cells were assessed by comet assay on days 1.5, 2.5, 3.5, and 4.375 of the preimplantation period of pregnancy. Main Outcome Measure(s) : Percentage of embryos and uterine cells with tail, mean comet tail length, percentage of fragmented DNA in tail. Result(s) : Significantly higher numbers of embryos with higher mean comet tail length and percentage of fragmented DNA in tail were observed in the LPS-treated compared with control animals as the period of pregnancy approaches the stage of implantation. At the same time, DNA damage was also significantly higher in the uterine cells of LPS-treated compared with control animals. Conclusion(s) : The MD of LPS can induce DNA damage in the preimplantation-stage embryos and uterine cells, which causes poor embryonic development and improper preparation of uterine horns during the preimplantation period of pregnancy, which may ultimately inhibit the process of implantation in mouse.

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