Lactoferrin is associated with a decrease in oocyte depletion in mice receiving cyclophosphamide  

Oleh:

Horiuchi, Yuka ; Nishiguchi, Toshihiro ; Tatsumi, Keiji ; Takakura, Kenji ; Fujii, Shingo ; Konishi, Ikuo

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 5 Sup (May 2009), page 2069-2078.
Topik: Cyclophosphamide; fertility preservation; lactoferrin; premature ovarian failure

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective : To investigate new important molecules involved in the regulation of chemotherapy-induced ovarian damage and, based on those results, to examine the effect of lactoferrin on cyclophosphamide (CPM)-induced ovarian failure. Design : Complementary DNA microarray and the administration of lactoferrin. Setting : Experimental animal study. Animal(s) : Female imprinting control region mice. Intervention(s) : Administration of CPM to mice, isolation of ovaries, isolation of RNA, microarray hybridization, and statistical analysis. According to the results of the microarray assay, administration of lactoferrin to CPM-treated mice, isolation of ovaries, isolation of RNA, and evaluation using quantitative polymerase chain reaction and histomorphometric analyses. Main Outcome Measure(s) : A list of nine down-regulated and two up-regulated genes with reliable hybridization signals was obtained. Several target molecules were then investigated. Result(s) : Among the listed genes, we focused on the mouse lactoferrin gene, because of its CPM-induced expression pattern and its multiple novel functions. Oral administration of bovine lactoferrin prevented down-regulation of the ovulation-related,Adamts1 and partial recovery of follicle depletion induced by CPM treatment. Conclusion(s) : The present report suggests that lactoferrin helps to rescue the ability to ovulate and partially to prevent oocyte depletion in mouse ovaries.

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