Effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent mouse embryo development  

Oleh:

Byung, Chul Jee ; Hai-Ying, Chen ; Ri-Cheng, Chian

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 5 Sup (May 2009), page 2037-2042.
Topik: In vitro maturation; immature oocytes; phosphodiesterase inhibitor; meiotic arrest; prematuration culture

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective : To investigate the effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent embryo development. Design : Animal study. Setting : University laboratory. Animal(s) : CD-1 mice. Intervention(s) : Immature oocytes were arrested meiotically in medium containing a specific phosphodiesterase type 3 inhibitor, cilostamide (1 µmol/L, 5 µmol/L, and 10 µmol/L), for 6 and 24 hours of prematuration culture, and then matured in vitro in inhibitor-free medium. Main Outcome Measure(s) : Oocyte maturation, fertilization, and early embryonic development in vitro. Result(s) : After 6 and 24 hours of prematuration culture with 1 µmol/L, 5 µmol/L, and 10 µmol/L cilostamide, there were no differences in maturation, fertilization, and early embryonic development compared with non-arrested controls. Conclusion(s) : Prematuration culture with cilostamide, for either 6 or 24 hours, may not be beneficial for subsequent embryonic development of immature mouse oocytes.

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