Insulin Resistance Directly Contributes to Androgenic Potential Within Ovarian Theca Cells  

Oleh:

JunWei, Qu ; Yong, Wang ; Xiaoke, Wu ; Lei, Gao ; Lihui, Hou ; Erkkola, Risto

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 5 Sup (May 2009), page 1990-1997.
Topik: Insulin resistance; ovarian theca cells; insulin sensitizers; PCOS

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective : To investigate whether insulin resistance (IR) within theca cells may directly contribute to their hyperandrogenism, a heritable trait of polycystic ovary syndrome (PCOS). Design : In vitro cell model. Setting : University-affiliated laboratory. Animal(s) : Porcine ovaries. Intervention(s) : Ovarian theca cells from porcine follicles were isolated and cultured. Insulin resistance was induced in theca cells without (Con) or with dexamethasone (Dex); cells were further treated by troglitazone (Tro) and metformin (Met) in IR cells or by vehicle only in IR and Con cells. Main Outcome Measure(s) : Medium glucose and T levels; reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot for insulin signal molecules and androgenic enzyme. Result(s) : As compared with Con cells, Dex-treated cells had significantly lower [3H]-glucose uptake (565 ± 58 cpm/106 vs. 1077 ± 78 cpm/106) but higher medium glucose levels (16.31 ± 0.39 nmol/L vs. 10.62 ± 1.02 nmol/L) and had approximately twofold T levels (0.82 ± 0.20 µg/L vs. 0.38±0.08µg/L). Troglitazone and Met significantly reduced the medium glucose and testosterone concentrations to levels comparable to those in Con cells. The RT-PCR and Western blot showed that the two sensitizers in different ways reversed the altered messenger RNA and protein expression of insulin receptor substrate-1, glucose transporter-4, peroxisome proliferator-activated receptor-?, and 17a-hydroxylase in Dex-induced IR cells. Conclusion(s) : Insulin resistance induced by Dex could directly exaggerate androgenic potential within theca cells, suggesting the possible involvement of this ovarian metabolic phenotype in PCOS hyperandrogenism.

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali