Antiacrosin antibodies and infertility. II. Gene immunization with human proacrosin to assess the effect of immunity toward proacrosin/acrosin upon protein activities and animal fertility  

Oleh:

Veaute, Carolina ; Furlong, Laura I. ; Cameo, Monica ; Harris, Jeffrey D. ; Vazquez-Levin, Monica H.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 04 (Apr. 2009), page 1256-1268.
Topik: Human; gene immunization; spermatozoa; proacrosin/acrosin system; antisperm antibodies; ZP binding and amidase activity; IVF and early embryonic development; fertility

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Objective : To assess the effect of antiacrosin antibodies upon proacrosin/acrosin activities and animal fertility. Design : Prospective study. Setting : Basic research laboratory. Patient(s) : A gene immunization (GI) model was developed; mice were injected with the sequence encoding human proacrosin (h-proacrosin), cloned in an expression vector. Intervention(s) : Subcloning of h-proacrosin in a eukaryotic expression vector (promoter, CMV; leader sequence, a-1 antitrypsin; pSF2-Acro); GI of female mice with this plasmid. Main Outcome Measure(s) : The following parameters were evaluated: [1] adequate conditions for GI protocols, [2] humoral response to GI with pSF2-Acro, [3] protein regions recognized by the antibodies, and [4] effect of antibodies upon proacrosin/acrosin–ZPA binding and amidase activity, and animal fertility. Result(s) : Conditions of female mice GI with the proacrosin sequence were established (plasmid purification with anion exchange chromatography and 40 µg of pSF2-Acro per dose) to trigger an immune response, reaching maximum levels at week 9 after the first injection. Antibodies produced by GI recognized human and mouse sperm acrosin systems, inhibited human proacrosin/acrosin interaction with recombinant human ZPA and protease activity, and negatively affected mouse IVF and early embryonic development. In addition, mice immunized with SF2-Acro exhibited a significantly lower size of fetuses. Conclusion(s) : Antiacrosin antibodies developed by using GI inhibit human proacrosin/acrosin activities and impair mouse fertility.

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