| DNA gyrase is the main target of Ciprofloxacin in Gram-negative bacteria such as Campylobacter. The most common mechanism found to be responsible for the development of Ciprofloxacin resistance is the transition of C to T in codon 86 of gyrA, which encodes one subunit of DNA gyrase. However, not all resistant isolates have mutation in codon 86. Mutation in other positions of gyrA might be responsible for this resistance. Sequencing of the whole gyrA gene was conducted to find other possible mutations. In this study, transition of G to A in codon 90 of gyrA was found to be responsible for Ciprofloxacin resistance of C. jejuni. Other transition occurred (A to G in codon 203; G to A in codon 285) seemed to be random mutations that were found in both resistant and susceptible isolates. |