Sperm head morphology is related to high deoxyribonucleic acid stainability assessed by sperm chromatin structure assay  

Oleh:

Zini, Armand ; Phillips, Simon ; Courchesne, Annick

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Fertility and Sterility (keterangan: ada di ClinicalKey) vol. 91 no. 06 (Jun. 2009), page 2495-2500.
Topik: Sperm; DNA fragmentation; nuclear condensation; sperm morphology; male infertility

Ketersediaan

Perpustakaan FK Nomor Panggil: F02.K.2009.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objective To examine the relationship between sperm strict morphology and sperm chromatin integrity. Design Prospective study. Setting Infertility clinic. Patient(s) Eighty-seven consecutive semen samples from non-azoospermic men presenting for infertility evaluation and 6 samples from fertile donors. Intervention(s) Assessment of standard semen parameters and sperm chromatin structure assay (SCSA) parameters (%DFI [DNA fragmentation index] and %HDS [high DNA stainability]). Evaluation of %HDS and %DFI after treatment with dithiothreitol (a thiol-reducing agent used to decondense sperm nuclei) was also undertaken. Main Outcome Measure(s) Relationship between sperm strict morphology defects and SCSA parameters (%DFI and %HDS). Result(s) We observed significant relationships between the percentage of normal sperm forms and both %HDS (r = -0.40) and sperm motility (r = 0.32). We also found significant relationships between sperm head defects and both %HDS (r = 0.40) and sperm concentration (r = -0.39). Sperm tail, midpiece, and neck defects were not significantly related to the SCSA parameters. Treatment of spermatozoa with dithiothreitol (to induce decondensation) resulted in a substantial increase in %HDS but no measurable change in %DFI. Conclusion(s) The observed relationship between sperm head defects and %HDS suggests that sperm head abnormalities may, in part, be due to incomplete sperm chromatin condensation.

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