The experience of two European preimplantation genetic diagnosis centres on human leukocyte antigen typing  

Oleh:

Velde, Hilde Van de ; Rycke, Martine De ; Man, Caroline De ; Hauwere, Kim De ; Fiorentino, Francesco

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: Human Reproduction vol. 24 no. 03 (Mar. 2009), page 732.
Topik: preimplantation HLA typing/preimplantation genetic diagnosis/HLA matching/short tandem repeats/live birth rate

Ketersediaan

Perpustakaan FK Nomor Panggil: H07.K.2009.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

BACKGROUND: Two European centres report on human leukocyte antigen (HLA) typing of preimplantation embryos for haematopoietic stem cell (HSC) transplantation: ‘UZ Brussel’ in Brussels and ‘Genoma’ in Rome. Both centres have 6 years’ experience with technical and clinical aspects of this type of genetic analysis on single blastomeres. METHODS: Both centres apply a similar technique for preimplantation HLA typing using short tandem repeats linked to the HLA locus in multiplex PCR for haplotyping. RESULTS: At present, a conclusive HLA diagnosis could be assured in 92.8% and 90.3% of the embryos at UZ Brussel and at Genoma, respectively. The implantation rates were 32.4% and 28.2%, respectively, and the birth rates per cycle were 9.4% and 18.6%, respectively. The HLA programme at UZ Brussel and at Genoma resulted in the birth of 9 babies and 3 successful HSC transplantations, and 42 babies and 7 successful HSC transplantations, respectively, so far. CONCLUSIONS: Drastic embryo selection for preimplantation HLA typing (in theory 1/4 for HLA, 1/8 for HLA in combination with sexing for X-linked recessive diseases, 3/16 for HLA in combination with autosomal recessive disorders) resulted overall in the birth of 51 babies (15.9% live birth rate per started cycle) in two European centres.

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