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BukuIsolation and Identification of Ice-Nucleating-Active Bacteria from Indonesian Edible Leafy Plant Poh-Pohan [Pilea glaberina] (Microbiology Indonesia, Vol. 2 No. 1, April 2008)
Bibliografi
Author: Waturangi, Diana Elizabeth ; Meicy, Vicky ; Suwanto, Antonius
Topik: Ice-Nucleating Bacteria; Indonesian Edible Leafy Plant; Pilea Glaberina
Bahasa: (EN )    
Penerbit: Fakultas Teknobiologi Unika Atma Jaya (Faculty of Biotechnology Atma Jaya Catholic University of Indonesia)     Tempat Terbit: Jakarta    Tahun Terbit: 2008    
Jenis: Article - diterbitkan di jurnal ilmiah nasional
Fulltext: Isolation and Identification of Ice-Nucleating-Active Bacteria from Indonesian.pdf (1.41MB; 5 download)
Abstract
Two ice-nucleating-active (rNA) bacteria (isolates C and 6) were isolated from poh-pohan (Pilea glaberina). an Indonesian edible leafy plant (lalaban). The maximum nucleation temperature of aqueous suspensions of the two isolates is -5 °C. They were classified as a type II ice nucleator. Microscopic and morphological determination showed that these isolates had yellow pigmentation, rod shape, and were Gram negative. Biochemical analysis indicated that the isolates were exhibited catalase activity, but negative in oxidase and indole assays. DNA sequencing of 16SrRNA gene of isolate A3 showed a 94% similarity to Pseiidomonas sp. while isolate A4 showed a 97% similarity to Xanthomonas campestris. To our knowledge, this is the first report of IN A bacteria isolated from a tropical edible leafy plant.
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