Detail Identification of Direct p73 Target Genes Combining DNA Microarray and Chromatin Immunoprecipitation Analyses (in The Journal of Biological Chemistry Vol. 277, No. 45) Bibliografi Author: Fontemaggi, Giulia ; Kela, Itai ; Amariglio, Ninette ; Rechavi, Gideon ; Krishnamurthy, Janakiraman ; Strano, Sabrina ; Sacchi, Ada ; Givol, David ; Blandino, Giovanni Topik: DNA Microarray; Chromatin Immunoprecipitation; p53 Tumor Suppressor Bahasa: (EN )    Edisi: Nov 2002     Penerbit: The American Society for Biochemistry and Molecular Biology (ASBMB)     Tempat Terbit: Maryland    Tahun Terbit: 2002     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: JBC 2002.pdf (437.78KB; 3 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract The newly discovered p53 family member, p73, has a striking homology to p53 in both sequence and modular structure. Ectopic expression of p73 promotes transcription of p53 target genes and recapitulates the most characterized p53 biological effects such as growth arrest, apoptosis, and differentiation. Unlike p53-deficient mice that develop normally but are subject to spontaneous tumor formation, p73-deficient mice exhibit severe defects in the development of central nervous system and suffer from inflammation but are not prone to tumor development. These phenotypes suggest different biological activities mediated by p53 and p73 that might reflect activation of specific sets of target genes. Here,we have analyzed the gene expression profile of H1299 cells after p73a or p53 activation using oligonucleotide microarrays capable of detecting ~11,000 mRNA species. Our results indicate that p73a and p53 activate both common and distinct groups of genes. We found 141 and 320 genes whose expression is modulated by p73a and p53, respectively. p73 a up-regulates 85 genes, whereas p53 induces 153 genes, of which 27 are in common with p73a. Functional classification of these genes reveals that they are involved in many aspects of cell function ranging from cell cycle and apoptosis to DNA repair. Furthermore, we report that some of the upregulated genes are directly activated by p73 or p53. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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