Detail A practical nucleic acid stain for quality control of printed DNA Microarrays Bibliografi Author: Wood, Jill G. ; Stephenson, Kate ; Beaudet, Matthew P. ; Cox, W. Gregory Topik: DNA Microarrays Bahasa: (EN )     Tahun Terbit: 2003     Jenis: Papers/Makalah - pada seminar internasional Fulltext: 353.pdf (314.26KB; 0 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract DNA microarray validation requires accurate evaluation of DNA quantity, spot morphology, spot uniformity, and identifi cation of missing features. Such assessment helps avoid under representation of genes in expression studies, wasted time, reagents, and valuable RNA. A nucleic acid stain enabling relative quantitation would be useful for microarray quality validation prior to hybridization. Currently available stains are not optimal for DNA printed on glass slides due to high, non-uniform background and inconsistent staining. A new dye was identifi ed which becomes fl uorescent upon binding either oligonucleotides or DNA printed on glass. This provides an optimal stain for DNA microarrays. The dye exhibits high sensitivity, detecting down to 1 picogram of DNA, and has a useful range of 1 to more than 100 picograms of DNA. The dye is compatible with all types of slide surfaces tested and with DNA spotted out of 50% DMSO, 3X SSC, PBS, and several commercially available spotting buffers Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

Copyright © 2006, 2007 Unika Atma Jaya, all rights reserved