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Concerns About Unreliable Data from Spotted cDNA Microarrays Due to Cross-Hybridization and Sequence Errors (in Statistical Applications in Genetics and Molecular Biology Vol 3(1))
Bibliografi
Author:
Handley, Daniel
;
Serban, Nicoleta
;
Peters, David G.
;
Glymour, Clark
Topik:
Cross-hybridization
;
Sequence Errors
;
cDNA microarray
;
Sources of Error
Bahasa:
(EN )
Edisi:
2004
Penerbit:
The Berkeley Electronic Press (bepress)
Tempat Terbit:
Berkeley
Tahun Terbit:
2004
Jenis:
Article - diterbitkan di jurnal ilmiah internasional
Fulltext:
handley2004a.pdf
(89.78KB;
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Abstract
We discuss our concerns regarding the reliability of data generated by spotted cDNA microarrays. Two types of error we highlight are cross-hybridization artifact due to sequence homologies and sequence errors in the cDNA used for spotting on microarrays. We feel that statisticians who analyze microarray data should be aware of these sources of unreliability intrinsic to cDNA microarray design and use.
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