Detail Transcriptional response of Saccharomyces cerevisiae to DNA-damaging agents does not identify the genes that protect against these agents (PNAS Vol.99 No.13) Bibliografi Author: Birrell, Geoff W. ; Brown, James A. ; Wu, H. Irene ; Giaever, Guri ; Chu, Angela M. ; Davis, Ronald W. ; Brown, J. Martin Topik: Yeast Strains; DNA-Damaging Agents; Gene Expression Profiling; PCR Amplification; Microarray Hybridization; Deletion Pool; Microarray Bahasa: (EN )    Edisi: Jun 2002     Penerbit: National Academy of Sciences     Tempat Terbit: Washington, D.C.    Tahun Terbit: 2002     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: Birrell.PNSA.2002.pdf (203.17KB; 0 download) Abstract The recent completion of the deletion of all of the nonessential genes in budding yeast has provided a powerful new way of determining those genes that affect the sensitivity of this organism to cytotoxic agents. We have used this system to test the hypoth¬esis that genes whose transcription is increased after DNA damage are important for the survival to that damage. We used a pool of 4,627 diploid strains each with homozygous deletion of a nones-sential gene to identify those genes that are important for the survival of yeast to four DNA-damaging agents: ionizing radiation, UV radiation, and exposure to cisplatin or to hydrogen peroxide. In addition we measured the transcriptional response of the wild-type parental strain to the same DNA-damaging agents. We found no relationship between the genes necessary for survival to the DNA-damaging agents and those genes whose transcription is increased after exposure. These data show that few, if any, of the genes involvedin repairing the DNA lesions produced in this study, including double-strand breaks, pyrimidine dimers, single-strand breaks, base damage, and DNA cross-links, are induced in response to toxic doses of the agents that produce these lesions. Thisfinding suggests that the enzymes necessary for the repair of these lesions are at sufficient levels within the cell. The data also suggest that the nature of the lesions produced by DNA-damaging agents cannot easily be deduced from gene expression profiling. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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