Detail Identification of Genes Modulated in Rheumatoid Arthritis Using Complementary DNA Microarray Analysis of Lymphoblastoid B Cell Lines From Disease-Discordant Monozygotic Twins (Arthritis & Rheumatism Vol. 54, No. 7) Bibliografi Author: Haas, Christian S. ; Creighton, Chad J. ; Pi, Xiujun ; Maine, Ira ; Koch, Alisa E. ; Haines III, G. Kenneth ; Ling, Song ; Chinnaiyan, Arul M. ; Holoshitz, Joseph Topik: Complementary DNA Microarray; cDNA Microarray; Rheumatoid Arthritis; RA; Lymphoblastoid B Cell Lines; LCLs Bahasa: (EN )    Edisi: Jul 2006     Penerbit: American College of Rheumatology     Tempat Terbit: Atlanta    Tahun Terbit: 2006     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 21953_ftp.pdf (313.73KB; 0 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Objective. To identify disease-specific gene expression profiles in patients with rheumatoid arthritis (RA), using complementary DNA (cDNA) microarray analyses on lymphoblastoid B cell lines (LCLs) derived from RA-discordant monozygotic (MZ) twins. Methods. The cDNA was prepared from LCLs derived from the peripheral blood of 11 pairs of RA-discordant MZ twins. The RA twin cDNA was labeled with cy5 fluorescent dye, and the cDNA of the healthy co-twin was labeled with cy3. To determine relative expression profiles, cDNA from each twin pair was combined and hybridized on 20,000-element microarray chips. Immunohistochemistry and real-time polymerase chain reaction were used to detect the expression of selected gene products in synovial tissue from patients with RA compared with patients with osteoarthritis and normal healthy controls. Results. In RA twin LCLs compared with healthy co-twin LCLs, 1,163 transcripts were significantly dif-ferentially expressed. Of these, 747 were overexpressed and 416 were underexpressed. Gene ontology analysis revealed many genes known to play a role in apoptosis, angiogenesis, proteolysis, and signaling. The 3 most significantly overexpressed genes were laeverin (a novel enzyme with sequence homology to CD13), 11/3-hydroxysteroid dehydrogenase type 2 (a steroid pathway enzyme), and cysteine-rich, angiogenic inducer 61 (a known angiogenic factor). The products of these genes, heretofore uncharacterized in RA, were all abundantly expressed in RA synovial tissues. Conclusion. Microarray cDNA analysis of peri-pheral blood-derived LCLs from well-controlled patient populations is a useful tool to detect RA-relevant genes and could help in identifying novel therapeutic targets. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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