Detail Specific differences in gene expression profile revealed by cDNA microarray analysis of glutathione 5-transferase placental form (GST-P) immunohistochemically positive rat liver foci and surrounding tissue (in Carcinogenesis vol.25 no.3) Bibliografi Author: Suzuki, Shugo ; Asamoto, Makoto ; Tsujimura, Kazunari ; Shirai, Tomoyuki Topik: Immunohistochemical; Glutamylcysteine Synthetase; GST-P; Heterocyclic Amine; HCA; cDNA Microarray Bahasa: (EN )    Edisi: 2004     Penerbit: Oxford University Press     Tempat Terbit: London    Tahun Terbit: 2004     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 439.pdf (107.93KB; 0 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Glutathione S-transferase placental form (GST-P), one of the glutathione S-transferases family of detoxification enzymes, is a very useful marker of rat liver pre-neoplastic lesions. We here investigated the gene expression profile in GST-P positive foci as compared with surrounding GST-P negative areas in the same liver of rats treated with diethyl-nitrosamine and then 2-acetylaminofluorene combined with partical hepatectomy. GST-P positive foci were har¬vested by laser microdissection and total RNAs were extracted to allow gene expression profiles to be assessed by cDNA microarray assays. Transaldolase, rat aflatoxin Bl aldehyde reductase and gamma-glutamylcysteine synthetase were found as up-regulated genes and regucal-cin as a down-regulated gene, in line with findings for hepatocellular carcinomas. The results indicate that the approach adopted is useful for understanding mechanisms of hepatocarcinogenesis and identification of new markers for rat liver pre-neoplastic foci. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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