Detail Controlling RNA self-assembly to form filaments (in Nucleic Acids Research, Vol. 34, No. 5) Bibliografi Author: Nasalean, Lorena ; Baudrey, Stephanie ; Leontis, Neocles B. ; Jaeger, Luc Topik: Nanotechnology; RNA tectonics; tectoRNAs Bahasa: (EN )    Edisi: Mar 2006     Penerbit: Oxford University Press     Tempat Terbit: London    Tahun Terbit: 2006     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 1381c.pdf (1.28MB; 0 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Fundamental control over supra-molecular selfassembly for organization of matter on the nanoscale is a major objective of nanoscience and nanotechnology. ‘RNA tectonics’ is the design of modular RNAunits, called tectoRNAs, that can beprogrammed to self-assemble into novel nano-and meso-scopic architectures of desired size and shape. We report the three-dimensional design of tectoRNAs incorporating modular 4-way junction (4WJ) motifs, hairpin loops and their cognate loop–receptors to create extended, programmable interaction inter-faces.Specific and directional RNA–RNAinteractions at these interfaces enable conformational, topological and orientational control of tectoRNA self-assembly. The interacting motifs are precisely positioned within the helical arms of the 4WJ to program assembly from only one helical stacking conformation of the 4WJ. TectoRNAs programmed to assemble with orientational compensation produce micrometer-scale RNA filaments through supra-molecular equilibrium polymerization. As visualized by transmission electron microscopy, these RNA filaments resemble actin filaments from the protein world. This work emphasizes the potential of RNA as a scaffold for designing and engineering new controllable biomaterials mimicking modern cytoskeletal proteins. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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