Detail Purification and Characterization of a Fibrinolytic Enzyme Produced from Bacillus sp. strain CK 11-4 Screened from Chungkook-Jang (in Applied and Environmental Microbiology Vol. 62, No. 7) Bibliografi Author: Kim, Wonkeuk (Co-Author); Choi, Keehyun ; Kim, Yongtaek ; Park, Hyunghwan ; Choi, Jangyoon ; Lee, Yoonsoo ; Oh, Hoonil ; Kwon, Ikboo ; Lee, Shinyoung Topik: Fibrinolytic Enzyme; Bacillus sp.; Chungkook-Jang; Fermented Food; Traditional Korean Fermented; Soybean Sauce Bahasa: (EN )    Edisi: Jul 1996     Penerbit: American Society for Microbiology     Tempat Terbit: Washington, D.C.    Tahun Terbit: 1996     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 9 Purification and Characterization of a Fibrinolytic Enzyme.pdf (360.6KB; 1 download) Abstract Bacillus sp. strain CK 11-4, which produces a strongly fibrinolytic enzyme, was screened from Chungkook-Jang, a traditional Korean fermented-soybean sauce. The fibrinolytic enzyme (CK) was purified from supernatant of Bacillus sp. strain CK 11-4 culture broth and showed thermophilic, hydrophilic, and strong fibrinolytic activity. The optimum temperature and pH were 70&C and 10.5, respectively, and the molecular weight was 28,200 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The first 14 amino acids of the N-terminal sequence of CK are Ala-Gln-Thr-Val-Pro-Tyr-Gly-Ile-Pro-Leu-Ile-Lys-Ala-Asp. This sequence is identical to that of subtilisin Carlsberg and different from that of nattokinase, but CK showed a level of fibrinolytic activity that was about eight times higher than that of subtilisin Carlsberg. The amidolytic activity of CK increased about twofold at the initial state of the reaction when CK enzyme was added to a mixture of plasminogen and substrate (H-D-Val-Leu-Lys-rNA). A similar result was also obtained from fibrin plate analysis. Related courses BIO 206 - PANGAN FUNG. DAN NUTRASEUTIKAL (Bacaan Anjuran) BIO 222 - TEKNOLOGI PANGAN (Bacaan Anjuran) BIP 455 - TEKNOLOGI PANGAN (Bacaan Anjuran) BIP 479 - TEK. FERMENTASI PANGAN TRAD. (Bacaan Anjuran) BIP 481 - PANGAN FUNGSI. & NUTRASEUTIKAL (Bacaan Anjuran) Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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