| Eleven Vibrw isolates obtained from a shrimp hatchery in Lampung, Indonesia and six reference isolates from CALM Mexico and ATCC USA were identified using Biolog System. They were isolated from morbid, healthy, dead shrimp or shrimp larvae. Biolog result showed that there were 5 species from 17 tested isolates based on their metabolic fingerprinting. Each of those species has unique metabolic pattern. Several isolates are dangerous for shrimp larvae based on the isolation and they were identified as V. alginolyticus by Biolog. Isolation protocol specifically for species is needed as an early detection, so that we can prevent distribution of this species in the hatchery. Based on Biolog result, formic acid and butyric acid are agents which could be utilized by V. aiginolyticus. In this study, we tried to use those agents and made a selective and differential medium based on modification of VP8 medium. Supplementation of formic acid in modified VP8 at pH 8.0 could not differentiate V. alginolyticus from V. proteolyticus, but supplementation of 250 mM butync acid at pH 8.0 can differentiate between those species. Moreover, 8 % NaCI in modified VP8 can inhibit other species of important marine J’ibrios (V. cholerae, V. Jiuvialis, V. vulnjficus, and V. parahaemolyticus). This medium showed great potential as primary isolation protocol and offers significant advantages over TCBS, ~TP8and ~THAagar for differentiating and selecting V. alginolyticusfrom other marine and estuanne Vibrio species. |