Detail Use of Multiplex PCR Method for Detection of ctxA and toxR gene of V. cholerae O1 and Non-o1 from Clinical Samples Bibliografi Author: DIRGANTARA, YANNI ; Lay, Bibiana Widiyati (Advisor) Topik: V. cholerae; cholera toxin; ctxA; toxR; multiplex PCR Bahasa: (EN )     Penerbit: Fakultas Teknobiologi Unika Atma Jaya (Faculty of Biotechnology Atma Jaya Catholic University of Indonesia)     Tempat Terbit: Jakarta    Tahun Terbit: 2008     Jenis: Theses - Undergraduate Thesis Fulltext: Yanni Dirgantara's Undergraduate Theses.pdf (601.19KB; 0 download) Ketersediaan Perpustakaan Pusat (Semanggi) Nomor Panggil: FTB-155 Non-tandon: tidak ada Tandon: 1  Lihat Detail Induk Abstract Three hundreds and seventy nine isolates of V. cholerae 01 strains and 30 isolates of V. cholerae Non-Ol strains from diarrhea suffering patients were identified at NAMRU-2. Two sets of specific primers were used for detection of clxA (564 bp) and toxR (779 bp) gene of all V. cholerae isolates using the multiplex PCR method. The result of this assay showed 362 isolates of V. cholerae 01 strains and I isolate of V. cholerae Non-Ol strains possessed the ctt~Avirulence gene. All isolates from both strains gave positive result for the presence of toxR regulatory gene. Sequencing method was used to see the similarity between the ctxA sequence of V. cholerae 01 and Non-Ol with the other ctxAsequence from the genebank. High similarity of 01 Indonesia (161532) and Non-Ol Indonesia (111954) strains and the other 16 strains of V. cholerae were described in the BLAST result. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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