Promoter sequences of 13 Phosphate Transporter genes of Oryza sativa (OsPT) have been analyzed in silico to identify their cis-regulatory elements (CREs). The DNA sequences of these OsPT genes were mined from NCBI gene databases. MEGABLAST program was used to align these sequences to Rice Genome Database in obtaining their complete sequences. The upstream region (-1 to -400) of the complete gene sequences were
analyzed using SIGNALSCAN program provided by PLACE database of cis-regulatory element motives. From this procedure, 153 types of CRE were identified. Four of these CREs were found on all of OsPT genes, which were ARR1AT, CAATBOX1, CACTFTPPCA1, and DOFCOREZM. Among these 4 CREs, CACTFTPPCA1 was found in 3 to 15 duplications on each of the cis-regulatory sequences. In addition, each OsPT gene has typical CREs that can only be found in the respective genes. The total number of these typical CREs is 54, and one of them was a binding site for bHLH-like protein, CACGTGMOTIF or the G-box. Moreover,
several E-boxes, which also functioned as a binding site for bHLH-like protein, were identified in all OsPTs except in OsPT1, OsPT6, and OsPT8. There were significant correlations (p < 0.05) between mRNA level of OsPT1 to OsPT11 in rice root with or without inoculation of Glomus intradices reported by Paszkowskidagger et al. (2002) and the
duplication numbers of ARR1AT, CAATBOX1, CACTFTPPCA1, CURECORECR, and
WRKY71OS. |