| Dengue virus (DV) has been a health concern over decades worldwide, including Indonesia. The infections may be asymptomatic, mild or severe manifestations that can cause fatalities. No antiviral or vaccine is known available against this virus. Virus isolation is important to acquire live viruses in order to support the development of antiviral or vaccine. It is also a gold standard for dengue virus diagnostic. However, conventional virus isolation applies a time consuming procedure. This was a preliminary study to apply flow cytometry for the development of sensitive, less time consuming virus isolation procedure. In this study, dendritic cells specific intercellular adhesion molecule 3 grabbing nonintegrin (DC-SIGN) transfected Raji (D-Raji) cells were compared with C6/36 cells that used in conventional virus isolation. Hyper-immune mouse ascetic fluid to Den-1 (HMAF) was used to detect Den-1 infection in direct and indirect assays. Direct detection using HMAF labeled Alexa 532 could detect infected D-Raji cells 20 hours post infection (hpi). Indirect detection using HMAF, a-mouse biotin, and streptavidin phycoerythrin can be an alternative choice for the detection. The application of D-Raji cells and flow cytometry for virus isolation may reduce time consuming procedure. However, further studies need to be done to optimize direct or indirect assays so that the detection of Den-2, Den-3, and Den-4 infection can be done simultaneously. And it is also necessary to determine the incubation time to detect D-Raji cells infected by DV from samples in order to make a diagnosis confirmation and to obtain good yield of live viruses. |