| Influenza A and B viruses are world-wide major causative agents of human acute respiratory infections. There are several influenza A and B strains circulating in Indonesia: A/H1N1/Hawaii, A/H1N1/New Caledonia, A/H1N1/Solomon, A/H3N2/Wisconsin, A/H3N2/New York, A/H3N2/California, B/Florida, B/Hawaii, B/Hong Kong, B/Ohio, B/Sichuan, and B/Shanghai. These twelve Influenza A and B strains were grown in Madin-Darby Canine Kidney (MDCK) cell line for seven days. Hemagglutination test (HAT) was performed every day to determine the viral growth in MDCK cell culture. The purpose of this study is to determine conditions that allow better isolation of these subtypes and strains. Influenza B/Florida, B/Sichuan and B/Ohio reached higher HA titer than influenza A strains. The HAT result for all influenza A/H3N2 did not show any significant increase HA titer. Influenza A/H1N1 reached a peak HA titer on day three, and then had a constant phase until day seven. Three of six influenza B strains had a constant phase then reached peak on the last day. Influenza B/Hong Kong and B/Hawaii had a similar growth curves with influenza A/H1N1. The HAT results showed that influenza B strains grew better than influenza A strains. Influenza A/H3N2/California, Influenza A/H3N2/Wisconsin, and Influenza A/H1N1/Solomon showed viral titers when trypsin was added. However, Influenza A/H3N2/New York and B/Shanghai still did not show viral titers |